Indigo is a tool to deconvolute sanger trace files with the help of a provided reference. Indigo calculates the sequence of two allels based on the mixed traces read sequencing both allels at the same time with one primer.
The trace files can be provided in abi or scf trace format (*.scf, *.abi, *.ab1, *.ab! and *.ab). The reference can be a genome selecte from the dropdown menu, an uploaded as a fasta file (*.fa) or a other trace file (*.scf, *.abi, *.ab1, *.ab! and *.ab).